Pub. Date : 1993 Feb 5
PMID : 8381406
16 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | The chondroitin sulfate moiety of thrombomodulin binds a second molecule of thrombin. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 2 | The role of the chondroitin sulfate moiety of thrombomodulin (TM) in the binding of thrombin to TM has been examined using fluorescent derivatives of thrombin. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 3 | When ANS-FPR-thrombin was titrated with TM lacking the chondroitin sulfate moiety (csf-TM), a monotonic and saturable increase in ANS emission intensity was observed that was consistent with the formation of a high affinity 1:1 thrombin-csf-TM complex. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 4 | In contrast, titration of ANS-FPR-thrombin with intact TM containing the chondroitin sulfate resulted in a biphasic change in ANS-FPR-thrombin emission intensity that was consistent with each molecule of TM binding at least two molecules of ANS-FPR-thrombin with different affinities. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 5 | In contrast, titration of ANS-FPR-thrombin with intact TM containing the chondroitin sulfate resulted in a biphasic change in ANS-FPR-thrombin emission intensity that was consistent with each molecule of TM binding at least two molecules of ANS-FPR-thrombin with different affinities. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 6 | In contrast, titration of ANS-FPR-thrombin with intact TM containing the chondroitin sulfate resulted in a biphasic change in ANS-FPR-thrombin emission intensity that was consistent with each molecule of TM binding at least two molecules of ANS-FPR-thrombin with different affinities. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 7 | This suggested that the second thrombin binds to TM via the chondroitin sulfate moiety. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 8 | A direct interaction between thrombin and chondroitin sulfate was demonstrated by showing that chondroitin sulfate, cleaved and purified from TM, caused a saturable increase in ANS emission intensity upon addition to an ANS-FPR-thrombin sample. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 9 | A direct interaction between thrombin and chondroitin sulfate was demonstrated by showing that chondroitin sulfate, cleaved and purified from TM, caused a saturable increase in ANS emission intensity upon addition to an ANS-FPR-thrombin sample. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 10 | A direct interaction between thrombin and chondroitin sulfate was demonstrated by showing that chondroitin sulfate, cleaved and purified from TM, caused a saturable increase in ANS emission intensity upon addition to an ANS-FPR-thrombin sample. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 11 | The minimum Kd for the ANS-FPR-thrombin-chondroitin sulfate complex was approximately 20 nM, consistent with chondroitin sulfate being the lower affinity binding site on TM for thrombin. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 12 | The minimum Kd for the ANS-FPR-thrombin-chondroitin sulfate complex was approximately 20 nM, consistent with chondroitin sulfate being the lower affinity binding site on TM for thrombin. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 13 | The minimum Kd for the ANS-FPR-thrombin-chondroitin sulfate complex was approximately 20 nM, consistent with chondroitin sulfate being the lower affinity binding site on TM for thrombin. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 14 | Thus, the chondroitin sulfate binds to thrombin somewhere other than anion-binding exosite I, and in doing so, alters the structure and/or environment of the active site more than 15A from the active site serine without detectably changing the conformation near Ser-195. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 15 | Since excess TM and excess csf-TM increased the ANS emission intensity of ANS-FPR-thrombin to different extents (approximately 15 and approximately 80%, respectively), the chondroitin sulfate also influences the environment of the active site probe even when thrombin is bound to the higher affinity site on TM (GF5-6). | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 16 | Since excess TM and excess csf-TM increased the ANS emission intensity of ANS-FPR-thrombin to different extents (approximately 15 and approximately 80%, respectively), the chondroitin sulfate also influences the environment of the active site probe even when thrombin is bound to the higher affinity site on TM (GF5-6). | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |