Title : Interleukin-1 selectively potentiates bradykinin-stimulated arachidonic acid release from human synovial fibroblasts.

Pub. Date : 1993 Jul

PMID : 8373725






11 Functional Relationships(s)
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1 Interleukin-1 selectively potentiates bradykinin-stimulated arachidonic acid release from human synovial fibroblasts. Arachidonic Acid kininogen 1 Homo sapiens
2 Exposure of human synovial fibroblasts prelabelled with [3H]arachidonic acid to bradykinin causes a rapid and sustained increase in arachidonic acid release, a transient increase in cytosolic calcium and an increase in radiolabelled diacylglycerol. Arachidonic Acid kininogen 1 Homo sapiens
3 Activation of arachidonic acid release by bradykinin was potentiated by interleukin-1 added either simultaneously with bradykinin or to cultures 24 h before addition of bradykinin. Arachidonic Acid kininogen 1 Homo sapiens
4 Activation of arachidonic acid release by bradykinin was potentiated by interleukin-1 added either simultaneously with bradykinin or to cultures 24 h before addition of bradykinin. Arachidonic Acid kininogen 1 Homo sapiens
5 Activation of arachidonic acid release by bradykinin was potentiated by interleukin-1 added either simultaneously with bradykinin or to cultures 24 h before addition of bradykinin. Arachidonic Acid kininogen 1 Homo sapiens
6 The stimulation of arachidonic acid release in response to bradykinin, in the absence or presence of interleukin-1, was not affected by RHC-80267, an inhibitor of diacylglycerol kinase, suggesting that deacylation of diacylglycerol was not an important pathway of arachidonic acid production in cultures exposed to bradykinin. Arachidonic Acid kininogen 1 Homo sapiens
7 The stimulation of arachidonic acid release in response to bradykinin, in the absence or presence of interleukin-1, was not affected by RHC-80267, an inhibitor of diacylglycerol kinase, suggesting that deacylation of diacylglycerol was not an important pathway of arachidonic acid production in cultures exposed to bradykinin. Arachidonic Acid kininogen 1 Homo sapiens
8 The stimulation of arachidonic acid release in response to bradykinin, in the absence or presence of interleukin-1, was not affected by RHC-80267, an inhibitor of diacylglycerol kinase, suggesting that deacylation of diacylglycerol was not an important pathway of arachidonic acid production in cultures exposed to bradykinin. Arachidonic Acid kininogen 1 Homo sapiens
9 Bradykinin-stimulated release of arachidonic acid was prevented by down-regulating protein kinase C by pretreatment with phorbol 12-myristate 13-acetate and was unaffected by inhibitors of protein synthesis actinomycin D or cycloheximide. Arachidonic Acid kininogen 1 Homo sapiens
10 On the other hand, interleukin-1 amplification of bradykinin-stimulated release of arachidonic acid was blocked by actinomycin D and cycloheximide. Arachidonic Acid kininogen 1 Homo sapiens
11 The results from this study point to activation of phospholipase A2 as the source of arachidonic acid in response to bradykinin. Arachidonic Acid kininogen 1 Homo sapiens