Pub. Date : 1994 Jun
PMID : 8187833
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Phorbol ester TPA rapidly prevents activation of p34cdc2 histone H1 kinase and concomitantly the transition from G2 phase to mitosis in synchronized HeLa cells. | Tetradecanoylphorbol Acetate | cyclin dependent kinase 1 | Homo sapiens |
| 2 | Treatment of HeLa cells (synchronized around the G2-M transition) with TPA (10(-7) M) has now been shown to induce an overall decrease of the histone H1 kinase activity associated with anti-p34cdc2 immunoprecipitates after about 20 to 30 min. | Tetradecanoylphorbol Acetate | cyclin dependent kinase 1 | Homo sapiens |
| 3 | However, p34cdc2 from cultures treated with TPA was more intensely stained by anti-phosphotyrosine antibodies than that of control cells, indicating that TPA treatment probably prevented the tyrosine dephosphorylation required for expression of the histone H1 kinase activity of the complex. | Tetradecanoylphorbol Acetate | cyclin dependent kinase 1 | Homo sapiens |
| 4 | However, p34cdc2 from cultures treated with TPA was more intensely stained by anti-phosphotyrosine antibodies than that of control cells, indicating that TPA treatment probably prevented the tyrosine dephosphorylation required for expression of the histone H1 kinase activity of the complex. | Tetradecanoylphorbol Acetate | cyclin dependent kinase 1 | Homo sapiens |
| 5 | The results indicate that TPA treatment of HeLa cultures rapidly stops the G2-M transition because it very rapidly prevents the p34cdc2/Cyclin B complex in G2 cells from developing histone H1 kinase activity. | Tetradecanoylphorbol Acetate | cyclin dependent kinase 1 | Homo sapiens |