Pub. Date : 1994 Apr 22
PMID : 8163479
5 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Thrombomodulin (TM) binds thrombin to form a complex that activates the plasma anticoagulant zymogen protein C. TM is an integral membrane glycoprotein that contains a chondroitin sulfate moiety. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 2 | Interaction with thrombin involves both the protein component of TM, specifically the growth factor-like repeats 4-6 (TM 4-6), and chondroitin sulfate. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 3 | Removal of chondroitin sulfate decreases the affinity for thrombin approximately 10-fold and shifts the Ca2+ dependence of protein C activation from simple saturation at > or = 500 microM Ca2+ to a distinct optimum at approximately 100 microM Ca2+. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 4 | At 0.27 mM Ca2+, TM containing chondroitin sulfate binds thrombin (Kd(app) = 0.3 nM) approximately 45 times tighter than meizothrombin des-fragment 1 (Kd(app) = 14 nM). | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |
| 5 | These studies suggest that occupancy of anion binding exosite 2 by either chondroitin sulfate or fragment 2 alters thrombin conformation resulting in the altered Ca2+ dependence of protein C activation. | Chondroitin Sulfates | coagulation factor II, thrombin | Homo sapiens |