Title : The upstream repression sequence from the yeast enolase gene ENO1 is a complex regulatory element that binds multiple trans-acting factors including REB1.

Pub. Date : 1994 Apr 1

PMID : 8144571






7 Functional Relationships(s)
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1 Cis-acting sequences that modulate ENO1 URS (upstream repression site) element activity were identified by base pair substitution mutagenesis. Peptichemio phosphopyruvate hydratase ENO1 Saccharomyces cerevisiae S288C
2 A binding site for the yeast REB1 protein was identified near the 5" terminus of the ENO1 URS element. Peptichemio phosphopyruvate hydratase ENO1 Saccharomyces cerevisiae S288C
3 Base substitution mutations within a second region of the ENO1 URS element caused a 38% loss of URS activity in vivo. Peptichemio phosphopyruvate hydratase ENO1 Saccharomyces cerevisiae S288C
4 Base substitution mutations within a second region of the ENO1 URS element caused a 38% loss of URS activity in vivo. Peptichemio phosphopyruvate hydratase ENO1 Saccharomyces cerevisiae S288C
5 Base substitution mutations within a third region near the 3" terminus of the ENO1 URS element caused a 70% loss of URS activity in vivo. Peptichemio phosphopyruvate hydratase ENO1 Saccharomyces cerevisiae S288C
6 Base substitution mutations within a third region near the 3" terminus of the ENO1 URS element caused a 70% loss of URS activity in vivo. Peptichemio phosphopyruvate hydratase ENO1 Saccharomyces cerevisiae S288C
7 These results showed that ENO1 URS element activity was modulated by multiple cis-acting sequences that bound distinct trans-acting factors. Peptichemio phosphopyruvate hydratase ENO1 Saccharomyces cerevisiae S288C