Title : The lipoxygenase activity of myoglobin. Oxidation of linoleic acid by the ferryl oxygen rather than protein radical.

Pub. Date : 1994 Mar 11

PMID : 8125933






2 Functional Relationships(s)
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1 The stereochemical results, supported by 18O-labeling studies, definitively rule out a significant role for singlet oxygen in the myoglobin-catalyzed, H2O2-dependent oxidation of linoleic acid. Hydrogen Peroxide myoglobin Physeter catodon
2 The myoglobin protein radical formed with H2O2 also plays no part in the reaction because the Km and Vmax values for the oxidation of linoleic acid are similar for native myoglobin and two mutants (K102Q/Y103F/Y146F/Y151F and H64V/K102Q/Y103F/Y146F/Y151F) with no tyrosine residues. Hydrogen Peroxide myoglobin Physeter catodon