Title : Studies on Z-Fraction. I. Isolation and partial characterization of low molecular weight ligand-binding protein from rat hepatic cytosol.

Pub. Date : 1975 Jun

PMID : 807304






2 Functional Relationships(s)
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1 Three factors have contributed to the subsequent resolution of the Z-fraction and partial characterization of that protein within the fraction with ligand-binding properties (Z-protein): (1) the use of hexachlorophene as ligand; (2) the inclusion of glycerol, 20%, during isolation to prevent aggregation and loss of binding-activity; and (3) the development of a charcoal binding assay. Hexachlorophene fatty acid binding protein 1 Rattus norvegicus
2 With use of the charcoal method, apparent dissociation constants for the interaction between Z-protein and hexachlorophene, bilirubin and L-thyroxine, were found to be 20, 50, and 350 muM, respectively. Hexachlorophene fatty acid binding protein 1 Rattus norvegicus