Pub. Date : 1995 Mar 10
PMID : 7890667
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Phospholipid vesicle binding experiments showed that these three cleavage-defective mutant forms of PLC delta 1 could specifically bind to phosphatidylinositol 4,5-bisphosphate (PIP2) with an affinity similar to that of wild type enzyme. | Phosphatidylinositol 4,5-Diphosphate | phospholipase C delta 1 | Homo sapiens |
| 2 | Phospholipid vesicle binding experiments showed that these three cleavage-defective mutant forms of PLC delta 1 could specifically bind to phosphatidylinositol 4,5-bisphosphate (PIP2) with an affinity similar to that of wild type enzyme. | Phosphatidylinositol 4,5-Diphosphate | phospholipase C delta 1 | Homo sapiens |
| 3 | Western blotting analysis of trypsin-treated enzyme-PIP2 complexes revealed that a 67-kDa major protein fragment survived trypsin digestion if the wild type enzyme, E341G, or H356L mutant PLC delta 1 was preincubated with 7.5 microM PIP2, whereas if it was preincubated with 80 microM PIP2, the size of major protein surviving was comparable to that of intact enzyme. | Phosphatidylinositol 4,5-Diphosphate | phospholipase C delta 1 | Homo sapiens |
| 4 | These observations suggest that PLC delta 1 can recognize PIP2 through a high affinity and a low affinity binding site and that residues Glu341 and His356 are not involved in either high affinity or low affinity PIP2 binding but rather are essential for the Ca(2+)-dependent cleavage activity of PLC. | Phosphatidylinositol 4,5-Diphosphate | phospholipase C delta 1 | Homo sapiens |