Title : Binding of the pyruvate dehydrogenase kinase to recombinant constructs containing the inner lipoyl domain of the dihydrolipoyl acetyltransferase component.

Pub. Date : 1995 Jan 13

PMID : 7822313






3 Functional Relationships(s)
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1 Using GSH-Sepharose to selectively bind GST constructs, tightly bound kinase was shown to rapidly transfer in a highly preferential way from intact E2 core to GST constructs containing the E2L2 domain rather than to ones containing only the E2L1 domain. Sepharose glutathione S-transferase kappa 1 Homo sapiens
2 Using GSH-Sepharose to selectively bind GST constructs, tightly bound kinase was shown to rapidly transfer in a highly preferential way from intact E2 core to GST constructs containing the E2L2 domain rather than to ones containing only the E2L1 domain. Sepharose glutathione S-transferase kappa 1 Homo sapiens
3 GST-E2L2-kinase complexes could be eluted from GSH-Sepharose with glutathione. Sepharose glutathione S-transferase kappa 1 Homo sapiens