Title : The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins.

Pub. Date : 1995 Apr 18

PMID : 7737110






5 Functional Relationships(s)
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1 The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins. Proline vasodilator stimulated phosphoprotein Homo sapiens
2 The recent molecular cloning of the vasodilator-stimulated phosphoprotein (VASP), an established in vivo substrate of cAMP- and cGMP-dependent protein kinases, revealed the presence of a proline-rich domain which prompted us to investigate a possible interaction with profilins. Proline vasodilator stimulated phosphoprotein Homo sapiens
3 The recent molecular cloning of the vasodilator-stimulated phosphoprotein (VASP), an established in vivo substrate of cAMP- and cGMP-dependent protein kinases, revealed the presence of a proline-rich domain which prompted us to investigate a possible interaction with profilins. Proline vasodilator stimulated phosphoprotein Homo sapiens
4 Here, we demonstrate that VASP is a natural proline-rich profilin ligand. Proline vasodilator stimulated phosphoprotein Homo sapiens
5 Moreover, VASP and a novel protein were specifically extracted from total cell lysates by profilin affinity chromatography and subsequently eluted either with poly-L-proline or a peptide corresponding to a proline-rich VASP motif. Proline vasodilator stimulated phosphoprotein Homo sapiens