Title : Surfactant protein A-polylysine conjugates for delivery of DNA to airway cells in culture.

Pub. Date : 1995 Jan

PMID : 7703285






9 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Surfactant protein A (SP-A) was modified by covalent linkage with polylysine of average M(r) 21 kD ([Lys]21kD-SP-A) and utilized to transfect human airway epithelial cells (H441) in vitro. Lysine surfactant protein A1 Homo sapiens
2 Surfactant protein A (SP-A) was modified by covalent linkage with polylysine of average M(r) 21 kD ([Lys]21kD-SP-A) and utilized to transfect human airway epithelial cells (H441) in vitro. Lysine surfactant protein A1 Homo sapiens
3 Surfactant protein A (SP-A) was modified by covalent linkage with polylysine of average M(r) 21 kD ([Lys]21kD-SP-A) and utilized to transfect human airway epithelial cells (H441) in vitro. Lysine surfactant protein A1 Homo sapiens
4 Transfection of H441 cells was more efficient with [Lys]21kD-SP-A than with polylysine-DNA or unmodified SP-A-DNA complexes. Lysine surfactant protein A1 Homo sapiens
5 Transfection with [Lys]21kD-SP-A was effective at a protein-to-DNA molar ratio of 400:1 and in the presence of an exogenous surfactant preparation, Survanta. Lysine surfactant protein A1 Homo sapiens
6 Transfection with [Lys]21kD-SP-A was reduced in the presence of unmodified SP-A consistent with the concept of a receptor mediated uptake of protein-DNA complexes. Lysine surfactant protein A1 Homo sapiens
7 Transfection with [Lys]21kD-SP-A was reduced in the presence of unmodified SP-A consistent with the concept of a receptor mediated uptake of protein-DNA complexes. Lysine surfactant protein A1 Homo sapiens
8 Increased transfection efficiency correlated with decreasing diameter of the [Lys]21kD-SP-A-DNA complexes, and these complexes bound to the cell surface and pseudopodia of H441 cells. Lysine surfactant protein A1 Homo sapiens
9 Cotransfection by [Lys]21kD-SP-A-DNA and [Lys]10kD-SP-B resulted in an additive level of reporter gene (CAT) expression. Lysine surfactant protein A1 Homo sapiens