Title : Heat sensitivity of bleomycin-sensitive CHO derivatives is not due to improper initialization of heat shock response.

Pub. Date : 1993 Mar-Apr

PMID : 7682247






9 Functional Relationships(s)
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1 The level of activated HSF binding to HSE was higher in cells treated with low doses (1 ng/ml) of bleomycin than in cells treated with 1 or 25 micrograms/ml bleomycin. Bleomycin interleukin 6 Homo sapiens
2 The binding of heat shock factor (HSF) in response to heat and varying concentrations of bleomycin in the four cell lines was examined using a gel shift assay and a synthetic heat shock element (HSE). Bleomycin interleukin 6 Homo sapiens
3 The binding of heat shock factor (HSF) in response to heat and varying concentrations of bleomycin in the four cell lines was examined using a gel shift assay and a synthetic heat shock element (HSE). Bleomycin interleukin 6 Homo sapiens
4 Heat (45 degrees C, 10 min) and exposure to 1 micrograms/ml bleomycin for 1 h at 37 degrees C induced similar levels of HSF binding in all four cell lines. Bleomycin interleukin 6 Homo sapiens
5 We also examined if bleomycin dose and the length of recovery from bleomycin treatment affected the induction of HSF binding. Bleomycin interleukin 6 Homo sapiens
6 We also examined if bleomycin dose and the length of recovery from bleomycin treatment affected the induction of HSF binding. Bleomycin interleukin 6 Homo sapiens
7 The level of activated HSF binding to HSE was higher in cells treated with low doses (1 ng/ml) of bleomycin than in cells treated with 1 or 25 micrograms/ml bleomycin. Bleomycin interleukin 6 Homo sapiens
8 The amount of activated HSF was directly proportional to the time elapsed since bleomycin treatment. Bleomycin interleukin 6 Homo sapiens
9 Our results therefore indicate no difference between CHO and its bleomycin-sensitive derivatives in the ability to initiate the heat shock response as determined by the production of activated HSF in response to either heat or bleomycin. Bleomycin interleukin 6 Homo sapiens