Title : Photoaffinity labeling of rhodopsin and bacteriorhodopsin.

Pub. Date : 1995 Sep-Oct

PMID : 7662862






1 Functional Relationships(s)
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1 Photoaffinity labeling with bovine rhodopsin using a retinal with a fixed 11-cis-ene cross-linked exclusively to Trp-265/Leu-266 in helix F, showing that the beta-ionone C-3 is close to helix F. Moreover, since these labeled amino acids are in the middle of helix F, while the Schiff-base linkage to Lys-296 at the other terminus of the chromophore is also in the middle of helix G, the chromophore lies horizontally near the center of the lipid bilayer. Leucine rhodopsin Bos taurus