Title : Influence of Hepes- and CO2/HCO(3-)-buffer on Ca2+ transients induced by TRH and elevated K+ in rat pituitary GH4C1 cells.

Pub. Date : 1995 Jul

PMID : 7589788






3 Functional Relationships(s)
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1 Influence of Hepes- and CO2/HCO(3-)-buffer on Ca2+ transients induced by TRH and elevated K+ in rat pituitary GH4C1 cells. HEPES thyrotropin releasing hormone Rattus norvegicus
2 In both buffers, addition of TRH induced a transient increase in [Ca2+]i which attained a significantly higher peak in Hepes (357 +/- 43 nM) when compared with values measured in the presence of CO2/HCO3- (184 +/- 21 nM). HEPES thyrotropin releasing hormone Rattus norvegicus
3 The TRH-evoked increase in IP3 was higher in magnitude in Hepes than in CO2/HCO3-, although the stimulated/basal ratio was not different between the two buffers. HEPES thyrotropin releasing hormone Rattus norvegicus