Title : Analysis of mast cell activation using diamine oxidase-gold enzyme-affinity ultrastructural cytochemistry.

Pub. Date : 1995 May-Jun

PMID : 7542108






4 Functional Relationships(s)
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1 We review a new technique--diamine oxidase (DAO)-gold ultrastructural enzyme-affinity labeling--which we developed to localize histamine in subcellular sites of mast cells. Histamine D-amino acid oxidase Homo sapiens
2 We review a new technique--diamine oxidase (DAO)-gold ultrastructural enzyme-affinity labeling--which we developed to localize histamine in subcellular sites of mast cells. Histamine D-amino acid oxidase Homo sapiens
3 The DAO-gold method showed that isolated human lung mast cells contained abundant histamine in their cytoplasmic granules, a conclusion which was verified by a large number of specificity controls. Histamine D-amino acid oxidase Homo sapiens
4 The DAO-gold method demonstrated histamine in the electron-dense granules of mast cells in these lesions, but little or no histamine was detected in the swollen, empty granules of mast cells undergoing piecemeal degranulation. Histamine D-amino acid oxidase Homo sapiens