Pub. Date : 1995 Sep 5
PMID : 7498312
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | High affinity [3H]bradykinin binding sites have been identified in human skin cryosections by in vitro autoradiography. | Tritium | kininogen 1 | Homo sapiens |
2 | Equilibrium binding studies were performed with increasing concentrations of [3H]bradykinin for 120 min in the presence of protease inhibitors at 4 degrees C. In saturation experiments a single class of high affinity binding sites was identified with a dissociation constant Kd of 1.2 +/- 0.8 nM (mean +/- S.E.M., n = 3) and a maximal binding capacity Bmax of 33 +/- 8 fmol [3H]bradykinin specifically bound/mg protein (mean +/- S.E.M., n = 3). | Tritium | kininogen 1 | Homo sapiens |
3 | Equilibrium binding studies were performed with increasing concentrations of [3H]bradykinin for 120 min in the presence of protease inhibitors at 4 degrees C. In saturation experiments a single class of high affinity binding sites was identified with a dissociation constant Kd of 1.2 +/- 0.8 nM (mean +/- S.E.M., n = 3) and a maximal binding capacity Bmax of 33 +/- 8 fmol [3H]bradykinin specifically bound/mg protein (mean +/- S.E.M., n = 3). | Tritium | kininogen 1 | Homo sapiens |