Title : Autoradiographic localization and characterization of bradykinin receptors in human skin.

Pub. Date : 1995 Sep 5

PMID : 7498312






3 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 High affinity [3H]bradykinin binding sites have been identified in human skin cryosections by in vitro autoradiography. Tritium kininogen 1 Homo sapiens
2 Equilibrium binding studies were performed with increasing concentrations of [3H]bradykinin for 120 min in the presence of protease inhibitors at 4 degrees C. In saturation experiments a single class of high affinity binding sites was identified with a dissociation constant Kd of 1.2 +/- 0.8 nM (mean +/- S.E.M., n = 3) and a maximal binding capacity Bmax of 33 +/- 8 fmol [3H]bradykinin specifically bound/mg protein (mean +/- S.E.M., n = 3). Tritium kininogen 1 Homo sapiens
3 Equilibrium binding studies were performed with increasing concentrations of [3H]bradykinin for 120 min in the presence of protease inhibitors at 4 degrees C. In saturation experiments a single class of high affinity binding sites was identified with a dissociation constant Kd of 1.2 +/- 0.8 nM (mean +/- S.E.M., n = 3) and a maximal binding capacity Bmax of 33 +/- 8 fmol [3H]bradykinin specifically bound/mg protein (mean +/- S.E.M., n = 3). Tritium kininogen 1 Homo sapiens