Title : In vitro insulin-stimulated conversion of [U-14C]glucose to 14CO2 by rat thymocytes.

Pub. Date : 1978 Mar

PMID : 743991






7 Functional Relationships(s)
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1 We conclude that insulin can enhance the formation of 14CO2 from [U-14C]glucose by thymocytes in vitro, and that this response may require the synthesis of one or more proteins. 14co2 insulin Bos taurus
2 In vitro insulin-stimulated conversion of [U-14C]glucose to 14CO2 by rat thymocytes. 14co2 insulin Bos taurus
3 Addition of bovine insulin to thymocytes from adrenalectomized rats resulted in stimulation of [U-14C]glucose conversion to 14CO2. 14co2 insulin Bos taurus
4 A significant enhancement of 14CO2 formation by insulin occurred by 30 min of incubation, and was consistently observed at an insulin concentration of 10(-8) M. The response to insulin was similar at 0.55 and 1.1 mM glucose, and was obtained at three cell concentrations (0.5, 1.0, 2.0 X 10(8) cells/ml). 14co2 insulin Bos taurus
5 A significant enhancement of 14CO2 formation by insulin occurred by 30 min of incubation, and was consistently observed at an insulin concentration of 10(-8) M. The response to insulin was similar at 0.55 and 1.1 mM glucose, and was obtained at three cell concentrations (0.5, 1.0, 2.0 X 10(8) cells/ml). 14co2 insulin Bos taurus
6 A significant enhancement of 14CO2 formation by insulin occurred by 30 min of incubation, and was consistently observed at an insulin concentration of 10(-8) M. The response to insulin was similar at 0.55 and 1.1 mM glucose, and was obtained at three cell concentrations (0.5, 1.0, 2.0 X 10(8) cells/ml). 14co2 insulin Bos taurus
7 Cycloheximide, at a level of 2.5 X 10(-5) M, suppressed [3H]leucine incorporation by 93% and inhibited the stimulation of 14CO2 formation by insulin. 14co2 insulin Bos taurus