Title : The identification of cytochrome P-450-LM2 synthesized in vitro from a rabbit liver polysomal mRNA template.

Pub. Date : 1981 Jan 29

PMID : 7213733






3 Functional Relationships(s)
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1 We demonstrate the in vitro synthesis of cytochrome P-450-LM2 (phenobarbital-inducible form of liver microsomal cytochrome P-450) from a rabbit liver polysomal mRNA template by specific immunoprecipitation of the product. Phenobarbital cytochrome P450 2B4 Oryctolagus cuniculus
2 In addition, we demonstrate that phenobarbital increases the amount of translatable mRNA for cytochrome P-450-LM2 but not for albumin, suggesting that phenobarbital has selective effects on the amount of available translatable mRNA or on mRNA biosynthesis. Phenobarbital cytochrome P450 2B4 Oryctolagus cuniculus
3 In addition, we demonstrate that phenobarbital increases the amount of translatable mRNA for cytochrome P-450-LM2 but not for albumin, suggesting that phenobarbital has selective effects on the amount of available translatable mRNA or on mRNA biosynthesis. Phenobarbital cytochrome P450 2B4 Oryctolagus cuniculus