Title : Radioenzymatic determination of epinephrine, norepinephrine and dopamine in 0.1 ml plasma samples: plasma catecholamine response to submaximal and near maximal exercise.

Pub. Date : 1980 Jun

PMID : 7000971






1 Functional Relationships(s)
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1 The assay consists basically of 1. conversion of epinephrine, norepinephrine, and dopamine into their respective methyl-derivates in the presence of catechol-O-methyltransferase and S-adenosylmethionine-[3H]methyl, 2. extraction of the methylated 3H labelled amines with diethyl ether, 3. separation by thin-layer chromatography, 4. measurement in a beta radiation scintillation counter. Norepinephrine catechol-O-methyltransferase Homo sapiens