Title : Acetylcholine and choline in neuronal tissue measured by HPLC with electrochemical detection.

Pub. Date : 1983 Jul

PMID : 6864218






2 Functional Relationships(s)
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1 The method is based on the separation of ACh and Ch by reverse-phase HPLC and mixing the effluent as it emerges from the column with acetylcholinesterase and Ch oxidase, which converts endogenous Ch and Ch produced by the hydrolysis of ACh to betaine and hydrogen peroxide. Acetylcholine acetylcholinesterase (Cartwright blood group) Homo sapiens
2 The method is based on the separation of ACh and Ch by reverse-phase HPLC and mixing the effluent as it emerges from the column with acetylcholinesterase and Ch oxidase, which converts endogenous Ch and Ch produced by the hydrolysis of ACh to betaine and hydrogen peroxide. Acetylcholine acetylcholinesterase (Cartwright blood group) Homo sapiens