Title : Purification of rat liver microsomal cytochrome P-450 by chromatography on immobilized adamantane with a new method of detergent removal.

Pub. Date : 1984

PMID : 6534377






1 Functional Relationships(s)
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1 A method for the isolation of liver microsomal cytochrome P-450 from phenobarbital-treated rats is described which is based on chromatography on 1-adamantane-carbonyl-aminohexyl-Sepharose 4B, subsequent ion exchange chromatography on DEAE-cellulose and detergent removal by adsorption on silica gel G. The method gives preparations with high electrophoretic homogeneity which correspond to a major phenobarbital-inducible form of rat liver microsomal cytochrome P-450. DEAE-Cellulose cytochrome P450, family 2, subfamily g, polypeptide 1 Rattus norvegicus