Pub. Date : 1984 Oct 10
PMID : 6480583
3 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In the presence of membrane and Ni2+, [3H]AVP has a half-life of 7 h. In the absence of Ni2+, the half-life of [3H]AVP is 1.2 h. Fractionation by high-pressure liquid chromatography of the supernatant from the incubation media not containing Ni2+ yields three peaks of radioactivity. | Nickel(2+) | arginine vasopressin | Rattus norvegicus |
| 2 | Although Ni2+, Co2+, benzamidine, and phenanthroline can prevent [3H]AVP degradation, only Ni2+ and, to a much lesser extent, Co2+, can potentiate specific [3H]AVP binding. | Nickel(2+) | arginine vasopressin | Rattus norvegicus |
| 3 | The results show that AVP-specific binding has properties which parallel its biological activity in behavioral assays; that, ultimately, proteolysis by membrane-bound peptidases inactivates AVP; and that Ni2+ acts both by preventing AVP breakdown and by potentiating specific binding. | Nickel(2+) | arginine vasopressin | Rattus norvegicus |