Title : In vitro degradation of gastric mucin. Carbohydrate side chains protect polypeptide core from pancreatic proteases.

Pub. Date : 1983 Mar

PMID : 6185390






3 Functional Relationships(s)
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1 By contrast, after removal of 50% of its carbohydrates, mucin was susceptible to pancreatic proteases: 50% of mucin protein became soluble in 80% vol/vol ethanol after 24-h incubation with alpha-chymotrypsin and elastase, and 24% of mucin protein became soluble in 80% vol/vol ethanol after 24-h incubation with trypsin; after elastase treatment, its elution from Sephadex G-200 was markedly retarded. Carbohydrates LOC100508689 Homo sapiens
2 We conclude that the carbohydrate side chains of hog gastric mucin glycoprotein protect the underlying polypeptide core from proteolysis and that degradation of the carbohydrate side chains by glycosidases from fecal bacteria renders the polypeptide core susceptible to pancreatic proteases. Carbohydrates LOC100508689 Homo sapiens
3 We conclude that the carbohydrate side chains of hog gastric mucin glycoprotein protect the underlying polypeptide core from proteolysis and that degradation of the carbohydrate side chains by glycosidases from fecal bacteria renders the polypeptide core susceptible to pancreatic proteases. Carbohydrates LOC100508689 Homo sapiens