Title : Fusion and phase separation monitored by lifetime changes of a fluorescent phospholipid probe.

Pub. Date : 1986 Mar 11

PMID : 3964657






1 Functional Relationships(s)
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1 Ca2+-induced fusion of phosphatidylserine vesicles served to test the method and was shown to have an exponential half-time of 7 s. Phase separation (between the phosphatidylserine head groups of bulk lipid and the phosphatidylcholine head groups of the probe) was monitored by DPHpPC under the same conditions used to follow lipid mixing due to fusion. Phosphatidylcholines carbonic anhydrase 2 Homo sapiens