Title : Binding of high density lipoprotein to cultured fibroblasts after chemical alteration of apoprotein amino acid residues.

Pub. Date : 1986 Jan 5

PMID : 3941085






4 Functional Relationships(s)
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1 Treatment of HDL3 with tetranitromethane (TNM), however, caused a large dose-dependent decrease in binding, with maximum inhibition at 3 mM. Tetranitromethane HDL3 Homo sapiens
2 Treatment of HDL3 with tetranitromethane (TNM), however, caused a large dose-dependent decrease in binding, with maximum inhibition at 3 mM. Tetranitromethane HDL3 Homo sapiens
3 Gradient gel electrophoresis, molecular sieve chromatography, and electron microscopy showed only minor changes in size distribution and shape of HDL3 particles after treatment with 3 mM TNM, but at higher TNM concentrations, coalescence and aggregation of particles was evident. Tetranitromethane HDL3 Homo sapiens
4 Treatment of HDL3 with 3 mM TNM affected neither its promotion of the low affinity (receptor-independent) cholesterol efflux from cells nor its ability to accept cholesterol from an albumin suspension, yet promotion of high affinity (receptor-dependent) cholesterol efflux from cells was abolished. Tetranitromethane HDL3 Homo sapiens