Title : Effects of estradiol and progesterone on immunoreactive forms of hypothalamic luteinizing hormone-releasing hormone.

Pub. Date : 1986

PMID : 3520372






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1 In order to investigate mechanisms underlying the ovarian steroid action on hypothalamic luteinizing hormone-releasing hormone (LHRH) neurons, LHRH and a higher immunoreactive molecular form (MW 1,800 daltons) of the decapeptide were immunoassayed with antibodies of different specificities in hypothalamic subcellular fractions, after molecular sieve filtration on Biogel P4 columns equilibrated with 0.2 N acetic acid containing 0.02% sodium azide. Steroids gonadotropin releasing hormone 1 Rattus norvegicus
2 In order to investigate mechanisms underlying the ovarian steroid action on hypothalamic luteinizing hormone-releasing hormone (LHRH) neurons, LHRH and a higher immunoreactive molecular form (MW 1,800 daltons) of the decapeptide were immunoassayed with antibodies of different specificities in hypothalamic subcellular fractions, after molecular sieve filtration on Biogel P4 columns equilibrated with 0.2 N acetic acid containing 0.02% sodium azide. Steroids gonadotropin releasing hormone 1 Rattus norvegicus
3 In order to investigate mechanisms underlying the ovarian steroid action on hypothalamic luteinizing hormone-releasing hormone (LHRH) neurons, LHRH and a higher immunoreactive molecular form (MW 1,800 daltons) of the decapeptide were immunoassayed with antibodies of different specificities in hypothalamic subcellular fractions, after molecular sieve filtration on Biogel P4 columns equilibrated with 0.2 N acetic acid containing 0.02% sodium azide. Steroids gonadotropin releasing hormone 1 Rattus norvegicus
4 The data suggest that ovarian steroids not only influence release of hypothalamic LHRH but also the processing of LHRH precursor forms. Steroids gonadotropin releasing hormone 1 Rattus norvegicus