Title : Affinity Purification of Ubiquitinated Proteins Using p62-Agarose to Assess Ubiquitination of Clade A PP2Cs.

Pub. Date : 2022

PMID : 35152379






1 Functional Relationships(s)
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1 In this chapter, we provide a detailed protocol to assess the ubiquitination of PP2CA, a clade A PP2C, mediated by BPMs using agarose-immobilised p62-derived ubiquitin-associated (UBA) domain, which efficiently binds ubiquitinated proteins. Sepharose nucleoporin 62 Homo sapiens