Title : Synthesis and Identification of Biologically Active Mono-Labelled FITC-Insulin Conjugate.

Pub. Date : 2022 Mar

PMID : 35006485






1 Functional Relationships(s)
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1 Human insulin (Actrapid ) was labelled with fluorescein isothiocyanate (FITC) and the synthesised conjugate identified using reverse phase high performance liquid chromatography (RP-HPLC) on a C18 column and a gradient method with mobile phase A containing 0.1% trifluoroacetic acid (TFA) in Millipore water and mobile phase B containing 90% Acetonitrile, 10% Millipore water and 0.1% TFA. acetonitrile insulin Homo sapiens