Title : A novel in vitro assay model developed to measure both extracellular and intracellular acetylcholine levels for screening cholinergic agents.

Pub. Date : 2021

PMID : 34637466






3 Functional Relationships(s)
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1 The associated changes in the cholinergic components may be reflected by intra- or extra-cellular ACh levels, with an increase in extracellular ACh levels occurring following AChE inhibition. Acetylcholine acetylcholinesterase (Cartwright blood group) Homo sapiens
2 RESULTS: Our cell-based assay system was capable of detecting increases in extracellular ACh levels induced by an AChE inhibitor at relatively high doses, as well as increases in intracellular ACh levels following the administration of lower AChE-inhibitor doses and an mAChR agonist. Acetylcholine acetylcholinesterase (Cartwright blood group) Homo sapiens
3 RESULTS: Our cell-based assay system was capable of detecting increases in extracellular ACh levels induced by an AChE inhibitor at relatively high doses, as well as increases in intracellular ACh levels following the administration of lower AChE-inhibitor doses and an mAChR agonist. Acetylcholine acetylcholinesterase (Cartwright blood group) Homo sapiens