Title : Distribution of catalase and its modulation by 12-O-tetradecanoylphorbol-13-acetate in murine dermis and subpopulations of keratinocytes differing in their stages of differentiation.

Pub. Date : 1988 Jul

PMID : 3383343






7 Functional Relationships(s)
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1 Distribution of catalase and its modulation by 12-O-tetradecanoylphorbol-13-acetate in murine dermis and subpopulations of keratinocytes differing in their stages of differentiation. Tetradecanoylphorbol Acetate catalase Mus musculus
2 Topical treatment of female SENCAR mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) reduced both dermal and epidermal catalase-specific activities 38% and 51% within 6 h and 18 h of promoter application, respectively. Tetradecanoylphorbol Acetate catalase Mus musculus
3 Topical treatment of female SENCAR mice with 12-O-tetradecanoylphorbol-13-acetate (TPA) reduced both dermal and epidermal catalase-specific activities 38% and 51% within 6 h and 18 h of promoter application, respectively. Tetradecanoylphorbol Acetate catalase Mus musculus
4 Pretreatment of the epidermis for 16-18 h with TPA (2 micrograms) uniformly reduced catalase-specific activity 46-52% in all keratinocyte subpopulations prepared by Percoll gradient centrifugation. Tetradecanoylphorbol Acetate catalase Mus musculus
5 Similarly, plots of catalase units per cell versus extracted protein per cell suggested 55-60% decreases in catalase activity in basal and spinous cell keratinocytes of TPA treated epidermis. Tetradecanoylphorbol Acetate catalase Mus musculus
6 Similarly, plots of catalase units per cell versus extracted protein per cell suggested 55-60% decreases in catalase activity in basal and spinous cell keratinocytes of TPA treated epidermis. Tetradecanoylphorbol Acetate catalase Mus musculus
7 Collectively, these studies suggest that: (i) TPA reduces the capacity for H2O2 detoxification by catalase throughout the epidermis; and (ii) activity measurements on unfractionated epidermal preparations may not be representative of the basal cell keratinocyte population. Tetradecanoylphorbol Acetate catalase Mus musculus