Title : Type of culture medium determines properties of cultivated retinal endothelial cells: induction of substantial phenotypic conversion by standard DMEM.

Pub. Date : 2021 Jan

PMID : 33521368






1 Functional Relationships(s)
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1 Within hours of cultivation in DMEM, the cell index - measured to assess the cell layer"s barrier function - dropped to ~5% of the initial value and only slowly recovered, not only accompanied by stronger expression of HSP70 mRNA and secretion of interleukin-6, but also by lower expressions of tight junction proteins claudin-5, claudin-1 or of the marker of cell type conversion caveolin-1. dmem claudin 1 Bos taurus