Title : A quantitative method for the detection and validation of catalase activity at physiological concentration in human serum, plasma and erythrocytes.

Pub. Date : 2021 Apr 15

PMID : 33486434






1 Functional Relationships(s)
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1 The method is based on the oxidation of pyrocatechol (PC) to give quinone form which by oxidative coupling with aminyl radical of 4-aminoantipyrine (4-AAP) resulting from H2O2/CAT to produce a pink colored quinone-imine product with lambdamax = 530 nm in a 100 mmol/L of tris buffer of pH 9.8 at room temperature (30 C). Hydrogen Peroxide catalase Homo sapiens