Title : A rapid and adaptable lipidomics method for quantitative UPLC-mass spectrometric analysis of phosphatidylethanolamine and phosphatidylcholine in vitro, and in cells.

Pub. Date : 2019 Apr 7

PMID : 31788037






5 Functional Relationships(s)
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1 To examine the substrate utilization of PE and PC by PLA2, we developed a method to accurately detect and measure specific forms of PE and PC as low as 50 femtomoles. Phosphatidylcholines phospholipase A2 group IB Homo sapiens
2 To examine the substrate utilization of PE and PC by PLA2, we developed a method to accurately detect and measure specific forms of PE and PC as low as 50 femtomoles. Phosphatidylcholines phospholipase A2 group IB Homo sapiens
3 Validation of this method consisted of an enzymatic assay to monitor docosahexaenoic acid and arachidonic acid release from the hydrolysis of PE and PC by group IV phospholipase A2 (cPLA2alpha) coupled to the generation of lyso-PE (LPE) and lyso-PC (LPC). Phosphatidylcholines phospholipase A2 group IB Homo sapiens
4 Validation of this method consisted of an enzymatic assay to monitor docosahexaenoic acid and arachidonic acid release from the hydrolysis of PE and PC by group IV phospholipase A2 (cPLA2alpha) coupled to the generation of lyso-PE (LPE) and lyso-PC (LPC). Phosphatidylcholines phospholipase A2 group IB Homo sapiens
5 This new UPLC ESI-MS/MS method provides accurate and highly sensitive detection of PE and PC species containing AA and DHA allowing for the specific examination of the substrate utilization of these phospholipids by PLA2 in vitro and in cells. Phosphatidylcholines phospholipase A2 group IB Homo sapiens