Title : Improving the heterologous expression of human β-defensin 2 (HBD2) using an experimental design.

Pub. Date : 2020 Mar

PMID : 31715251






1 Functional Relationships(s)
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1 After the evaluation of 19 different combination, the best condition to express HBD2 had a pre-induction temperature of 37 C, a cell density of 1.0 U (600 nm), an induction temperature of 20 C and a 0.1 mM of gene expression inducer (IPTG) over four hours. Isopropyl Thiogalactoside defensin beta 4A Homo sapiens