Pub. Date : 2019 Dec 19
PMID : 31612222
11 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Deletion of cytochrome P450 oxidoreductase enhances metabolism and DNA adduct formation of benzo[a]pyrene in Hepa1c1c7 cells. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 2 | The environmental carcinogen benzo[a]pyrene (BaP) is presumed to exert its genotoxic effects after metabolic activation by cytochrome P450 (CYP) enzymes. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 3 | The environmental carcinogen benzo[a]pyrene (BaP) is presumed to exert its genotoxic effects after metabolic activation by cytochrome P450 (CYP) enzymes. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 4 | The environmental carcinogen benzo[a]pyrene (BaP) is presumed to exert its genotoxic effects after metabolic activation by cytochrome P450 (CYP) enzymes. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 5 | The environmental carcinogen benzo[a]pyrene (BaP) is presumed to exert its genotoxic effects after metabolic activation by cytochrome P450 (CYP) enzymes. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 6 | However, studies using the Hepatic Reductase Null (HRN) mouse model, in which cytochrome P450 oxidoreductase (POR), the electron donor to CYP enzymes, is deleted specifically in hepatocytes, have shown that loss of hepatic POR-mediated CYP function leads to greater BaP-DNA adduct formation in livers of these mice than in wild-type (WT) mice. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 7 | In contrast, CYP-catalysed BaP-DNA adduct levels were ~10-fold higher in POR KO Hepa1c1c7 cells than in WT Hepa1c1c7 cells, in concordance with the presence of higher levels of BaP metabolite (e.g. BaP-7,8-dihydrodiol) in the medium of cultured BaP-exposed POR KO Hepa1c1c7 cells. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 8 | In contrast, CYP-catalysed BaP-DNA adduct levels were ~10-fold higher in POR KO Hepa1c1c7 cells than in WT Hepa1c1c7 cells, in concordance with the presence of higher levels of BaP metabolite (e.g. BaP-7,8-dihydrodiol) in the medium of cultured BaP-exposed POR KO Hepa1c1c7 cells. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 9 | In contrast, CYP-catalysed BaP-DNA adduct levels were ~10-fold higher in POR KO Hepa1c1c7 cells than in WT Hepa1c1c7 cells, in concordance with the presence of higher levels of BaP metabolite (e.g. BaP-7,8-dihydrodiol) in the medium of cultured BaP-exposed POR KO Hepa1c1c7 cells. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 10 | In contrast, CYP-catalysed BaP-DNA adduct levels were ~10-fold higher in POR KO Hepa1c1c7 cells than in WT Hepa1c1c7 cells, in concordance with the presence of higher levels of BaP metabolite (e.g. BaP-7,8-dihydrodiol) in the medium of cultured BaP-exposed POR KO Hepa1c1c7 cells. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |
| 11 | These results indicate that CYP enzymes may play a more important role in the detoxication of BaP, as opposed to its bioactivation. | Benzo(a)pyrene | cytochrome P450, family 21, subfamily a, polypeptide 1 | Mus musculus |