Pub. Date : 1987 Apr
PMID : 3104072
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
1 | Supplementation of the cells with exogenous GM1, but not GD1a, identified a larger population of cells adherent on CTB (comparable to pFN-adherent cells) and dramatically increased the proportion of cells capable of forming neurites without reducing the time requirement. | G(M1) Ganglioside | chitobiase | Homo sapiens |
2 | In ultrastructural studies using the scanning electron microscope (SEM) and immunofluorescence (IF) analyses to discriminate microtubule distributions, neurites of GM1-supplemented cells on CTB were virtually identical with pFN-adherent neurites, whereas unsupplemented cells on CTB generated processes with fine-structural differences. | G(M1) Ganglioside | chitobiase | Homo sapiens |
3 | Treatment of cells during the GM1 supplementation period with cycloheximide completely abolished the ability of cells to generate neurites on CTB and decreased the adhesive capacity of cells as well; a similar treatment of cells had no adverse effect on adherence or neurite extension on pFN. | G(M1) Ganglioside | chitobiase | Homo sapiens |
4 | The importance of one or more proteins in GM1-dependent processes was further confirmed by demonstrating the trypsin sensitivity of a cell surface component(s) required to achieve maximal attachment on CTB; in contrast, adherence and neurite extension on pFN were much more resistant to this treatment process. | G(M1) Ganglioside | chitobiase | Homo sapiens |