Title : Assessment of electrocatalytic hydroxylase activity of cytochrome P450 3A4 (CYP3A4) by means of derivatization of 6β-hydroxycortisol by sulfuric acid for fluorimetric assay.

Pub. Date : 2019 May 1

PMID : 30683357






1 Functional Relationships(s)
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1 We have shown that CYP3A4 substrate - hydrocortisone - and its 6beta-hydroxylated product have different emission wavelengths at an excitation lambdaex = 365 nm after treatment with sulfuric acid:ethanol (3:1) mixture (lambdaem = 525 +- 2 nm and lambdaem = 427 +- 2 nm, respectively). Hydrocortisone cytochrome P450 family 3 subfamily A member 4 Homo sapiens