Title : Highly sensitive colorimetric detection of glucose through glucose oxidase and Cu2+-catalyzed 3,3',5,5'-tetramethylbenzidine oxidation.

Pub. Date : 2019 Apr 15

PMID : 30677737






4 Functional Relationships(s)
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1 We develop a glucose oxidase (GOx)-mediated strategy for detecting glucose based on oxidized 3,3",5,5"-tetramethylbenzidine (oxTMB), which is generated from Cu2+-catalyzed 3,3",5,5"-tetramethylbenzidine (TMB)-H2O2 reaction, as colorimetric readout. Hydrogen Peroxide hydroxyacid oxidase 1 Homo sapiens
2 We develop a glucose oxidase (GOx)-mediated strategy for detecting glucose based on oxidized 3,3",5,5"-tetramethylbenzidine (oxTMB), which is generated from Cu2+-catalyzed 3,3",5,5"-tetramethylbenzidine (TMB)-H2O2 reaction, as colorimetric readout. Hydrogen Peroxide hydroxyacid oxidase 1 Homo sapiens
3 The sensing system involves two processes: generation of H2O2 from GOx-catalyzed oxidation of glucose, and H2O2-induced the oxidization of TMB via the catalysis of Cu2+. Hydrogen Peroxide hydroxyacid oxidase 1 Homo sapiens
4 The H2O2 formed by GOx-catalyzed oxidation of glucose oxidizes colorless TMB to blue oxTMB, thus enhancing the absorbance intensity at 670 nm. Hydrogen Peroxide hydroxyacid oxidase 1 Homo sapiens