Pub. Date : 1985 Feb
PMID : 2981664
4 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | In order to determine whether cAMP mediates the effects of PTH on the metabolism of 25-hydroxyvitamin D3 (25-OH-D3) on chick kidney cells in primary culture, the effect of forskolin on the production of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] and 24,25-dihydroxyvitamin D3 [24,25-(OH)2D3] was assessed. | Calcifediol | parathyroid hormone | Gallus gallus |
| 2 | Similar results were observed when cultures were preincubated with nonradioactive 25-OH-D3 for 4 h in the presence of PTH and forskolin, followed by a 30-min incubation with radioactive substrate. | Calcifediol | parathyroid hormone | Gallus gallus |
| 3 | At maximal concentrations of PTH (10 ng/ml) and forskolin (10 microM), the effects of the two on 25-OH-D3 metabolism were not additive. | Calcifediol | parathyroid hormone | Gallus gallus |
| 4 | The results of these studies indicate that the effects of PTH on the metabolism of 25-OH-D3 by chick kidney cells are mediated by cAMP, since they can be enhanced and mimicked by forskolin, that they are exerted at the level of both 1- and 24-hydroxylase activity, and that they are not dependent on the calcium concentration of the medium. | Calcifediol | parathyroid hormone | Gallus gallus |