Pub. Date : 2018 Feb
PMID : 29273683
7 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Glycosylation of human CBG influences its steroid-binding activity, and there are N-glycosylation sites in the reactive center loops (RCLs) of human and rat CBGs. | Steroids | serpin family A member 6 | Homo sapiens |
| 2 | Proteolysis of the RCL of human CBG causes a structural change that disrupts steroid binding. | Steroids | serpin family A member 6 | Homo sapiens |
| 3 | We now show that mutations of conserved N-glycosylation sites at N238 in human CBG and N230 in rat CBG disrupt steroid binding. | Steroids | serpin family A member 6 | Homo sapiens |
| 4 | Deglycosylation of fully glycosylated human CBG or human CBG with only one N-glycan at N238 with Endo H-reduced steroid-binding affinity, while PNGase F-mediated deglycosylation does not, indicating that steroid binding is preserved by deamidation of N238 when its N-glycan is removed. | Steroids | serpin family A member 6 | Homo sapiens |
| 5 | Deglycosylation of fully glycosylated human CBG or human CBG with only one N-glycan at N238 with Endo H-reduced steroid-binding affinity, while PNGase F-mediated deglycosylation does not, indicating that steroid binding is preserved by deamidation of N238 when its N-glycan is removed. | Steroids | serpin family A member 6 | Homo sapiens |
| 6 | When expressed in N-acetylglucosaminyltransferase-I-deficient Lec1 cells, human and rat CBGs, and a human CBG mutant with only one glycosylation site at N238, have higher (2-4 fold) steroid-binding affinities than when produced by sialylation-deficient Lec2 cells or glycosylation-competent CHO-S cells. | Steroids | serpin family A member 6 | Homo sapiens |
| 7 | Thus, the presence and composition of an N-glycan in this conserved position both appear to influence the steroid binding of CBG. | Steroids | serpin family A member 6 | Homo sapiens |