Title : New reporter gene assays for detecting natural and synthetic molting hormone agonists using yeasts expressing ecdysone receptors of various insects.

Pub. Date : 2017 Jul

PMID : 28680812






3 Functional Relationships(s)
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1 Synthetic nonsteroidal ecdysone agonists, a class of insect growth regulators (IGRs), target the ecdysone receptor (EcR), which forms a heterodimer with ultraspiracle (USP) to transactivate ecdysone response genes. Ecdysone ultraspiracle Drosophila melanogaster
2 In the present study, we developed reporter gene assay (RGA) systems to detect molting hormone (ecdysone) activity by introducing EcR-USP cDNA and a bacterial lacZ reporter gene into yeast. Ecdysone ultraspiracle Drosophila melanogaster
3 Furthermore, the insect order-selective ligand activities of synthetic nonsteroidal ecdysone agonists were linearly related to their binding activities, which were measured against in vitro translated EcR-USP complexes. Ecdysone ultraspiracle Drosophila melanogaster