Title : MicroRNA-27a/b mediates endothelin-1-induced PPARĪ³ reduction and proliferation of pulmonary artery smooth muscle cells.

Pub. Date : 2017 Sep

PMID : 28484848






5 Functional Relationships(s)
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1 Endothelin-1 (ET-1) dose- and time-dependently resulted in PPARgamma reduction and proliferation of primary cultured rat PASMC, which was accompanied by the activation of nuclear factor-kappaB (NF-kappaB) and subsequent induction of microRNA-27a/b (miR-27a/b) expression. pasmc microRNA 27a Rattus norvegicus
2 Endothelin-1 (ET-1) dose- and time-dependently resulted in PPARgamma reduction and proliferation of primary cultured rat PASMC, which was accompanied by the activation of nuclear factor-kappaB (NF-kappaB) and subsequent induction of microRNA-27a/b (miR-27a/b) expression. pasmc microRNA 27a Rattus norvegicus
3 Further study indicated that the presence of either NF-kappaB inhibitor pyrrolidinedithiocarbamate or prior silencing miR-27a/b with anti-miRNA oligonucleotides suppressed ET-1-induced PPARgamma reduction and proliferation of PASMC, while overexpression of miR-27a/b reduced PPARgamma expression and enhanced PASMC proliferation. pasmc microRNA 27a Rattus norvegicus
4 Further study indicated that the presence of either NF-kappaB inhibitor pyrrolidinedithiocarbamate or prior silencing miR-27a/b with anti-miRNA oligonucleotides suppressed ET-1-induced PPARgamma reduction and proliferation of PASMC, while overexpression of miR-27a/b reduced PPARgamma expression and enhanced PASMC proliferation. pasmc microRNA 27a Rattus norvegicus
5 Taken together, our study demonstrates that ET-1 stimulates miR-27a/b expression by activation of the NF-kappaB pathway, which in turn results in PPARgamma reduction and contributes to ET-1-induced PASMC proliferation. pasmc microRNA 27a Rattus norvegicus