Title : An In Vitro TORC1 Kinase Assay That Recapitulates the Gtr-Independent Glutamine-Responsive TORC1 Activation Mechanism on Yeast Vacuoles.

Pub. Date : 2017 Jul 15

PMID : 28483912






1 Functional Relationships(s)
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1 Using this system, we found that this mechanism specifically responds to l-glutamine, resides on the vacuolar membranes, and involves a previously uncharacterized Vps34-Vps15 phosphatidylinositol (PI) 3-kinase complex and the PI-3-phosphate [PI(3)P]-binding FYVE domain-containing vacuolar protein Pib2. phosphatidylinositol 3-phosphate Pib2p Saccharomyces cerevisiae S288C