Title : Drug-enhanced carbon monoxide production from heme by cytochrome P450 reductase.

Pub. Date : 2017 Jan-Mar

PMID : 28480030






1 Functional Relationships(s)
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1 Brain microsomes of Sprague-Dawley rats or recombinant human cytochrome P450 reductase (CPR) were incubated with NADPH and various drugs in closed vials in phosphate buffer at pH 7.4 and 37 C. After 15 minutes, the reaction was stopped by cooling in dry ice, and the headspace gas was analyzed for CO production using gas chromatography with reductive (mercuric oxide) detection. NADP cytochrome p450 oxidoreductase Homo sapiens