Pub. Date : 2017 Mar
PMID : 28338196
8 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Study on the proliferation of human gastric cancer cell AGS by activation of EGFR in H2O2. | Hydrogen Peroxide | epidermal growth factor receptor | Homo sapiens |
| 2 | The expression of the epidermal growth factor receptor (EGFR) and its downstream signaling pathway extracellular signal-regulated kinase (ERK) protein in H2O2 was detected by Western blot method; moreover, the effect of H2O2 on intracellular reactive oxygen species (ROS) in AGS cells was observed under the fluorescence microscope and quantitative analysis by flow cytometry. | Hydrogen Peroxide | epidermal growth factor receptor | Homo sapiens |
| 3 | The expression of the epidermal growth factor receptor (EGFR) and its downstream signaling pathway extracellular signal-regulated kinase (ERK) protein in H2O2 was detected by Western blot method; moreover, the effect of H2O2 on intracellular reactive oxygen species (ROS) in AGS cells was observed under the fluorescence microscope and quantitative analysis by flow cytometry. | Hydrogen Peroxide | epidermal growth factor receptor | Homo sapiens |
| 4 | The expression of the epidermal growth factor receptor (EGFR) and its downstream signaling pathway extracellular signal-regulated kinase (ERK) protein in H2O2 was detected by Western blot method; moreover, the effect of H2O2 on intracellular reactive oxygen species (ROS) in AGS cells was observed under the fluorescence microscope and quantitative analysis by flow cytometry. | Hydrogen Peroxide | epidermal growth factor receptor | Homo sapiens |
| 5 | The expression of the epidermal growth factor receptor (EGFR) and its downstream signaling pathway extracellular signal-regulated kinase (ERK) protein in H2O2 was detected by Western blot method; moreover, the effect of H2O2 on intracellular reactive oxygen species (ROS) in AGS cells was observed under the fluorescence microscope and quantitative analysis by flow cytometry. | Hydrogen Peroxide | epidermal growth factor receptor | Homo sapiens |
| 6 | The Western blot results showed that after 48 hours of low concentration of H2O2 treatment of AGS cells, the EGFR protein levels and ERK protein phosphorylation levels increased significantly (p<0.05). | Hydrogen Peroxide | epidermal growth factor receptor | Homo sapiens |
| 7 | RT-PCR results showed the levels of c-myc mRNA in AGS cells treated with a low concentration of H2O2 were significantly increased (p<0.05) and EGFR blocking agent AG1478 can resist the above effects. | Hydrogen Peroxide | epidermal growth factor receptor | Homo sapiens |
| 8 | CONCLUSIONS: Low concentration of H2O2 can significantly promote the proliferation of AGS cells by activating EGFR/ERK signaling pathway. | Hydrogen Peroxide | epidermal growth factor receptor | Homo sapiens |