Title : CRISPR/Cas9 mediated sequential editing of genes critical for ookinete motility in Plasmodium yoelii.

Pub. Date : 2017 Mar

PMID : 28034675






2 Functional Relationships(s)
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Compound Name
Protein Name
Organism
1 Drug resistant genes encoding human dihydrofolate reductase (hDHFR) and a yeast bifunctional protein (yFCU), with cytosine deaminase (CD) and uridyl phosphoribosyl transferase (UPRT) activities in the plasmid, allowed sequential positive (pyrimethamine, Pyr) and negative (5-fluorocytosine, 5FC) selections and generation of transgenic parasites free of the episomal plasmid after genetic modification. Flucytosine dihydrofolate reductase Homo sapiens
2 Drug resistant genes encoding human dihydrofolate reductase (hDHFR) and a yeast bifunctional protein (yFCU), with cytosine deaminase (CD) and uridyl phosphoribosyl transferase (UPRT) activities in the plasmid, allowed sequential positive (pyrimethamine, Pyr) and negative (5-fluorocytosine, 5FC) selections and generation of transgenic parasites free of the episomal plasmid after genetic modification. Flucytosine dihydrofolate reductase Homo sapiens