Title : An improved in vitro method for screening toxin and medicine targeting CYP2E1.

Pub. Date : 2016 Oct

PMID : 27653210






2 Functional Relationships(s)
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1 The results showed that in homogenate-mitochondrial method, the protein level and activity of CYP2E1 were increased by ethanol and pyrazole; reduced by diallyldisulfide and kaempferol, and this homogenate-mitochondrial method is convenient with good repeatability and reproducibility in screening chemicals targeting CYP2E1, especially for the inducers. pyrazole cytochrome P450, family 2, subfamily e, polypeptide 1 Mus musculus
2 The results showed that in homogenate-mitochondrial method, the protein level and activity of CYP2E1 were increased by ethanol and pyrazole; reduced by diallyldisulfide and kaempferol, and this homogenate-mitochondrial method is convenient with good repeatability and reproducibility in screening chemicals targeting CYP2E1, especially for the inducers. pyrazole cytochrome P450, family 2, subfamily e, polypeptide 1 Mus musculus