Pub. Date : 2017 Apr
PMID : 27604968
9 Functional Relationships(s)Download |
Sentence | Compound Name | Protein Name | Organism |
| 1 | Restoration of miR-1305 relieves the inhibitory effect of nicotine on periodontal ligament-derived stem cell proliferation, migration, and osteogenic differentiation. | Nicotine | microRNA 1305 | Homo sapiens |
| 2 | miR-1305 upregulation and its potential target RUNX2 downregulation exist in the PDLSCs exposed to nicotine. | Nicotine | microRNA 1305 | Homo sapiens |
| 3 | In this study, we aimed to investigate whether nicotine inhibits PDLSC proliferation, migration, and osteogenic differentiation by increasing miR-1305 level and decreasing RUNX2 level. | Nicotine | microRNA 1305 | Homo sapiens |
| 4 | METHODS: Quantitative real-time PCR (qRT-PCR) and Western blot assays were performed to detect the expression levels of miR-1305 and RUNX2 in the PDLSCs exposed to nicotine, respectively. | Nicotine | microRNA 1305 | Homo sapiens |
| 5 | RESULTS: Nicotine promoted miR-1305 expression and inhibited RUNX2 expression in PDLSCs. | Nicotine | microRNA 1305 | Homo sapiens |
| 6 | Cell proliferation, migration, and differentiation detection showed that nicotine suppressed proliferation, migration, and osteogenic differentiation of PDLSCs, and restoration of miR-1305 relieved the inhibitory effect of nicotine on PDLSCs. | Nicotine | microRNA 1305 | Homo sapiens |
| 7 | Moreover, we identified and validated that RUNX2 was a direct target of miR-1305, and upregulation of RUNX2 had similar effects with the downregulation of miR-1305 on relieving the inhibitory effect of nicotine on PDLSCs. | Nicotine | microRNA 1305 | Homo sapiens |
| 8 | Moreover, we identified and validated that RUNX2 was a direct target of miR-1305, and upregulation of RUNX2 had similar effects with the downregulation of miR-1305 on relieving the inhibitory effect of nicotine on PDLSCs. | Nicotine | microRNA 1305 | Homo sapiens |
| 9 | CONCLUSION: Nicotine suppresses proliferation, migration, and osteogenic differentiation of PDLSCs, and restoration of miR-1305 relieves the inhibitory effect of nicotine on PDLSCs depending on its target RUNX2. | Nicotine | microRNA 1305 | Homo sapiens |