Title : Rapid LC-MS/MS method for the determination of 4-hydroxycholesterol/cholesterol ratio in serum as endogenous biomarker for CYP3A activity in human and foals.

Pub. Date : 2016 Oct 15

PMID : 27565568






3 Functional Relationships(s)
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1 Rapid LC-MS/MS method for the determination of 4-hydroxycholesterol/cholesterol ratio in serum as endogenous biomarker for CYP3A activity in human and foals. cholest-5-ene-3,4-diol cytochrome P450 family 3 subfamily A member 4 Homo sapiens
2 Thus, it was the aim of this study to develop and validate an analytical method allowing estimation of the hepatic CYP3A enzyme activity using the 4-hydroxycholesterol to cholesterol ratio as an endogenous biomarker in serum. cholest-5-ene-3,4-diol cytochrome P450 family 3 subfamily A member 4 Homo sapiens
3 Finally, the validated assay was applied to measure 4-hydroxycholesterol and cholesterol in serum samples of clinical studies in humans and foals that could verify induction of hepatic CYP3A4 (human) and CYP3A89 (foals) after premedication with the known enzyme inducer rifampicin. cholest-5-ene-3,4-diol cytochrome P450 family 3 subfamily A member 4 Homo sapiens