Title : Identification of the cytochrome P-450 isozymes responsible for testosterone oxidation in rat lung, kidney, and testis: evidence that cytochrome P-450a (P450IIA1) is the physiologically important testosterone 7 alpha-hydroxylase in rat testis.

Pub. Date : 1989 Aug

PMID : 2752981






2 Functional Relationships(s)
Download
Sentence
Compound Name
Protein Name
Organism
1 Kidney microsomes from mature male rats were shown by Western immunoblot to contain cytochrome P-450h (P450IIC11), which has been shown previously to convert testosterone to 2 alpha-hydroxytestosterone, 16 alpha-hydroxytestosterone, and androstenedione. Testosterone cytochrome P450, subfamily 2, polypeptide 11 Rattus norvegicus
2 Antibody against cytochrome P-450h completely inhibited (greater than 95%) the 2 alpha- and 16 alpha-hydroxylation of testosterone by kidney microsomes, but had little effect on androstenedione formation, which is catalyzed by 17 beta-hydroxysteroid dehydrogenase. Testosterone cytochrome P450, subfamily 2, polypeptide 11 Rattus norvegicus